Genome-wide DNA promoter methylation and transcriptome analysis in human adipose tissue unravels novel candidate genes for obesity.


OBJECTIVE/METHODS: DNA methylation plays an important role in obesity and related metabolic complications. We examined genome-wide DNA promoter methylation along with mRNA profiles in paired samples of human subcutaneous adipose tissue (SAT) and omental visceral adipose tissue (OVAT) from non-obese vs. obese individuals. RESULTS: We identified negatively correlated methylation and expression of several obesity-associated genes in our discovery dataset and in silico replicated ETV6 in two independent cohorts. Further, we identified six adipose tissue depot-specific genes (HAND2, HOXC6, PPARG, SORBS2, CD36, and CLDN1). The effects were further supported in additional independent cohorts. Our top hits might play a role in adipogenesis and differentiation, obesity, lipid metabolism, and adipose tissue expandability. Finally, we show that in vitro methylation of SORBS2 directly represses gene expression. CONCLUSIONS: Taken together, our data show distinct tissue specific epigenetic alterations which associate with obesity.

PubMed ID: 28123940

Projects: LHA - Leipzig Health Atlas

Publication type: Not specified

Journal: Mol Metab

Human Diseases: Obesity

Citation: Mol Metab. 2016 Nov 16;6(1):86-100. doi: 10.1016/j.molmet.2016.11.003. eCollection 2017 Jan.

Date Published: 27th Jan 2017

Registered Mode: by PubMed ID

Authors: M. Keller, L. Hopp, X. Liu, T. Wohland, K. Rohde, R. Cancello, M. Klos, K. Bacos, M. Kern, F. Eichelmann, A. Dietrich, M. R. Schon, D. Gartner, T. Lohmann, M. Dressler, M. Stumvoll, P. Kovacs, A. M. DiBlasio, C. Ling, H. Binder, M. Bluher, Y. Bottcher

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Created: 22nd Apr 2020 at 13:11

Last updated: 7th Dec 2021 at 17:58

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