Data files

Meibomian glands (MGs), specialized sebaceous glands embedded in the eyelids, are an integral component of the ocular defense system, as their oily secretion product, meibum, is essential for protecting the eye surface. In spite of its importance for ocular health, the transcriptional changes that lead to meibum production by MG acinar cells (meibocytes) have not been characterized in a systematic way so far. By combining spatial transcriptomics (ST), pseudotime, and functional enrichment analysis, ...

The lipid-secreting sebaceous glands (SG) are indispensable for maintaining skin structure and function, and may influence whole body’s energy metabolism. SG deregulation is also associated with a number of skin disorders, including but not limited to acne. Here, we exploit the high cellular resolution provided by sebaceous hyperplasia to spatially characterize the human SG transcriptional program. By integrating single-cell spatial transcriptomics, pseudotime analysis, RNA velocity, and functional ...