Skin single-cell transcriptomics reveals a core of sebaceous gland-relevant genes shared by mice and humans
OverviewBackground Single-cell RNA sequencing (scRNAseq) has been widely applied to dissect cellular heterogeneity in normal and diseased skin. Sebaceous glands, essential skin components with established functions in maintaining skin integrity and emerging roles in systemic energy metabolism, have been largely neglected in scRNAseq studies. Methods Departing from mouse and human skin scRNAseq datasets, we identified gene sets expressed especially in sebaceous glands with the open-source R-package oposSOM. Results The identified gene sets included sebaceous gland-typical genes as Scd3, Mgst1, Cidea, Awat2 and KRT7. Surprisingly, however, there was not a single overlap among the 100 highest, exclusively in sebaceous glands expressed transcripts in mouse and human samples. Notably, both species share a common core of only 25 transcripts, including mitochondrial and peroxisomal genes involved in fatty acid, amino acid, and glucose processing, thus highlighting the intense metabolic rate of this gland. Conclusions This study highlights intrinsic differences in sebaceous lipid synthesis between mice and humans, and indicates an important role for peroxisomal processes in this context. Our data also provides attractive starting points for experimentally addressing novel candidates regulating sebaceous gland homeostasis. Keywords Sebaceous gland, skin, single-cell transcriptomics, bioinformatics
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LHA ID: 8HEHMJK77T-2
Public web page: Not specified
Human Diseases: No Human Disease specified
Health Atlas - Local Data Hub/Leipzig PALs: No PALs for this Project
Project Coordinators: No Project coordinators for this Project
Project created: 27th Apr 2023
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Author: Torsten Thalheim
Date Published: No date defined
Publication Type: Journal article
Cheng JB, Sedgewick AJ, Finnegan AI, Harirchian P, Lee J, Kwon S, Fassett MS, Golovato J, Gray M, Ghadially R, Liao W, Perez White BE, Mauro TM, Mully T, Kim EA, Sbitany H, Neuhaus IM, Grekin RC, Yu SS, Gray JW, Purdom E, Paus R, Vaske CJ, Benz SC, Song JS, Cho RJ. Transcriptional Programming of Normal and Inflamed Human Epidermis at Single-Cell Resolution. Cell Rep. 2018 Oct 23;25(4):871-883. doi: 10.1016/j.celrep.2018.09.006. PMID: 30355494; PMCID: PMC6367716.
European Genome-Phenome Archive ...
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Cheng JB, Sedgewick AJ, Finnegan AI, Harirchian P, Lee J, Kwon S, Fassett MS, Golovato J, Gray M, Ghadially R, Liao W, Perez White BE, Mauro TM, Mully T, Kim EA, Sbitany H, Neuhaus IM, Grekin RC, Yu SS, Gray JW, Purdom E, Paus R, Vaske CJ, Benz SC, Song JS, Cho RJ. Transcriptional Programming of Normal and Inflamed Human Epidermis at Single-Cell Resolution. Cell Rep. 2018 Oct 23;25(4):871-883. doi: 10.1016/j.celrep.2018.09.006. PMID: 30355494; PMCID: PMC6367716.
European Genome-Phenome Archive ...
Group Overexpression Spot J from Cheng oposSOM. Most of the samples in this spot are SG cells
Group Overexpression Spot K from Joost oposSOM. Most of the samples in this spot are SG cells
Joost S, Zeisel A, Jacob T, Sun X, La Manno G, Lönnerberg P, Linnarsson S, Kasper M. Single-Cell Transcriptomics Reveals that Differentiation and Spatial Signatures Shape Epidermal and Hair Follicle Heterogeneity. Cell Syst. 2016 Sep 28;3(3):221-237.e9. doi: 10.1016/j.cels.2016.08.010. Epub 2016 Sep 15. PMID: 27641957; PMCID: PMC5052454
GEO the accession number: GSE67602
Extended table 3 including Ensembl identifier of peroxisomal transcripts (GO:0005777) upregulated in human and mouse sebaceous gland cells.
Extended table 1 including Ensembl identifier for transcripts jointly upregulated in human and mouse sebaceous gland cells.
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Projects: funny dummy, Skin single-cell transcriptomics reveals a core of sebaceous gland-relevant genes shared by mice and humans
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